Rapid detection of hepatitis C virus RNA by a reverse transcription loop-mediated isothermal amplification assay
نویسندگان
چکیده
منابع مشابه
Reverse transcription-loop-mediated isothermal amplification assay for rapid detection of hepatitis E virus.
The one-step single-tube betaine-free reverse transcription (RT)-loop-mediated isothermal amplification assay was developed for rapid diagnosis of hepatitis E virus. This assay amplified the target gene in less than 45 min (even as short as 20 min) under isothermal conditions at 63 degrees C, and the sensitivity of this assay was 100-fold greater than that of RT-PCR. This assay demonstrated a d...
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Bovine viral diarrhea virus (BVDV) is a pathogen that infects cattle, and is globally important. It causes substantial financial losses to the livestock industry. In the current study, a one-step reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP) assay was set up for rapid and efficient detection of BVDV. For this purpose, four primers were designed to recognize six distinct...
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ژورنال
عنوان ژورنال: FEMS Immunology & Medical Microbiology
سال: 2011
ISSN: 0928-8244,1574-695X
DOI: 10.1111/j.1574-695x.2011.00828.x